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KMID : 1161520000040010031
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Animal Cells and Systems
2000 Volume.4 No. 1 p.31 ~ p.37
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Genetic differentiation among the mitochondrial nd2 gene and trnaTrp gene sequences of genus rana (anura) in Korea
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Lee Hyuk
Yang Suh-Yung
Lee Hei-Yung
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Abstract
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The genetic variations among six species of Rana from Korea (R. nigro?maculata, R. plancyi, R. dybowskii, R. sp, R. rugosa type A, B and R. amurensis) were investigated using 499 bases of mitochondrial DNA sequences for ND2 (NADH dehydrogenase subunit 2) gene and tRNATrp gene. Partial sequences of ND2 gene (427 bp) and full sequences of tRNATrp gene (73 bp) were identified. The level of sequence divergences ranged from 0.2 to 5.2% within species and 4.9?28.0% among 6 species of the genus Rana. The tRNATrp gene of the genus Rana was composed of 77 nucleotides which showed a two dimensional “cloverleaf” structure. The secondary structure of tRNATrp was not found compensatory changes which could potentially confound phylogenetic inference. In the neighbor?joining tree, brown frogs were clustered first with the level of sequence divergence of 13.20% between R. amurensis and R. dybowskii, and 9% between R. dybowskii and R. sp. supported by 99% bootstrap iterations, respectively. R. nigromaculata and R. plancyi were clustered into another group with 5.1% divergence supported by 100% bootstrap iteration. R. rugosa A and B types were grouped by 4.9% divergence and clustered into the last group with other two groups with 100% bootstrap iterations.
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KEYWORD
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Mt ND2 gene, Mt tRNATrp gene, Genus Rana, Genetic differentiation, Amphibia
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